connect qpcr application software Search Results


bio rad cfx connect real time pcr detection system  (Bio-Rad)
98
Bio-Rad bio rad cfx connect real time pcr detection system
Bio Rad Cfx Connect Real Time Pcr Detection System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/connect+qpcr+application+software/CFX+Manager+Software/pm39987671-148-11-18
Average 98 stars, based on 1 article reviews
bio rad cfx connect real time pcr detection system - by Bioz Stars, 2026-11
98/100 stars
  Buy from Supplier
cfx connect real time pcr system  (Bio-Rad)
98
Bio-Rad cfx connect real time pcr system
Cfx Connect Real Time Pcr System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/connect+qpcr+application+software/CFX+Maestro+Software/pm33581254-134-36-41
Average 98 stars, based on 1 article reviews
cfx connect real time pcr system - by Bioz Stars, 2026-11
98/100 stars
  Buy from Supplier
cfx connect real time pcr detection system  (Bio-Rad)
99
Bio-Rad cfx connect real time pcr detection system
Cfx Connect Real Time Pcr Detection System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/connect+qpcr+application+software/CFX+Connect+Real-Time+PCR+Detection+System+Firmware+Update/pm34608736-63-9-18
Average 99 stars, based on 1 article reviews
cfx connect real time pcr detection system - by Bioz Stars, 2026-11
99/100 stars
  Buy from Supplier
bio rad qpcr software  (Bio-Rad)
96
Bio-Rad bio rad qpcr software
Bio Rad Qpcr Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/connect+qpcr+application+software/ChromLab+Software/pmc08342130-27-18-21
Average 96 stars, based on 1 article reviews
bio rad qpcr software - by Bioz Stars, 2026-11
96/100 stars
  Buy from Supplier
cfx96 connect real time pcr system instrument  (Bio-Rad)
99
Bio-Rad cfx96 connect real time pcr system instrument
Cfx96 Connect Real Time Pcr System Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/connect+qpcr+application+software/CFX96+and+CFX384+Real-Time+PCR+Detection+Systems+Firmware+Update/pmc11634826-97-6-5
Average 99 stars, based on 1 article reviews
cfx96 connect real time pcr system instrument - by Bioz Stars, 2026-11
99/100 stars
  Buy from Supplier
connective tissue growth factor ctgf  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology connective tissue growth factor ctgf
FIGURE 1 YAP increases hTERT expression. A and B, MCF7 cells were transiently transfected with YAP-WT, YAP-5SA expression vectors or empty vector (Vec) as a control. Relative mRNA levels were examined by qPCR, and protein levels were detected by western blot. As a well-established YAP target gene, <t>CTGF</t> was used as a positive control. C and D, qPCR analysis of relative mRNA levels, and western blot analysis of protein levels in YAP-WT, YAP-5SA, or empty vector transfected HEK293A cells. E and F, WT and LATS1/2 KO HEK293A cells were transfected with control siRNA (siNC) or siRNAs against YAP (siYAP-1, siYAP-2) for 48 h. The mRNA levels were analyzed by qPCR, and protein levels were determined by western blot. Values represent mean ± SEM from three independent experiments. *P < .05, **P < .01, ***P < .001 by one-way ANOVA (A and C) or two-way ANOVA (E) with Fisher's LSD test. Relative hTERT protein levels were quantified with ImageJ software and normalized to β-actin, as indicated at the bottom of the blot
Connective Tissue Growth Factor Ctgf, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/connect+qpcr+application+software/CTGF+Antibody/pm31950551-19-36-42
Average 96 stars, based on 1 article reviews
connective tissue growth factor ctgf - by Bioz Stars, 2026-11
96/100 stars
  Buy from Supplier
powergene9600 plus real-time pcr system  (Atila Biosystems)
90
Atila Biosystems powergene9600 plus real-time pcr system
FIGURE 1 YAP increases hTERT expression. A and B, MCF7 cells were transiently transfected with YAP-WT, YAP-5SA expression vectors or empty vector (Vec) as a control. Relative mRNA levels were examined by qPCR, and protein levels were detected by western blot. As a well-established YAP target gene, <t>CTGF</t> was used as a positive control. C and D, qPCR analysis of relative mRNA levels, and western blot analysis of protein levels in YAP-WT, YAP-5SA, or empty vector transfected HEK293A cells. E and F, WT and LATS1/2 KO HEK293A cells were transfected with control siRNA (siNC) or siRNAs against YAP (siYAP-1, siYAP-2) for 48 h. The mRNA levels were analyzed by qPCR, and protein levels were determined by western blot. Values represent mean ± SEM from three independent experiments. *P < .05, **P < .01, ***P < .001 by one-way ANOVA (A and C) or two-way ANOVA (E) with Fisher's LSD test. Relative hTERT protein levels were quantified with ImageJ software and normalized to β-actin, as indicated at the bottom of the blot
Powergene9600 Plus Real Time Pcr System, supplied by Atila Biosystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/connect+qpcr+application+software/powergene9600+software++v1+0+14rc/pm37772799-67-12-19
Average 90 stars, based on 1 article reviews
powergene9600 plus real-time pcr system - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
cfx connect real time pcr machine biorad bio rad cfx96 real time pcr detection system  (Bio-Rad)
96
Bio-Rad cfx connect real time pcr machine biorad bio rad cfx96 real time pcr detection system
FIGURE 1 YAP increases hTERT expression. A and B, MCF7 cells were transiently transfected with YAP-WT, YAP-5SA expression vectors or empty vector (Vec) as a control. Relative mRNA levels were examined by qPCR, and protein levels were detected by western blot. As a well-established YAP target gene, <t>CTGF</t> was used as a positive control. C and D, qPCR analysis of relative mRNA levels, and western blot analysis of protein levels in YAP-WT, YAP-5SA, or empty vector transfected HEK293A cells. E and F, WT and LATS1/2 KO HEK293A cells were transfected with control siRNA (siNC) or siRNAs against YAP (siYAP-1, siYAP-2) for 48 h. The mRNA levels were analyzed by qPCR, and protein levels were determined by western blot. Values represent mean ± SEM from three independent experiments. *P < .05, **P < .01, ***P < .001 by one-way ANOVA (A and C) or two-way ANOVA (E) with Fisher's LSD test. Relative hTERT protein levels were quantified with ImageJ software and normalized to β-actin, as indicated at the bottom of the blot
Cfx Connect Real Time Pcr Machine Biorad Bio Rad Cfx96 Real Time Pcr Detection System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/connect+qpcr+application+software/CFX96+Touch+Real-Time+PCR+Detection+System+with+Starter+Package/pm37913774-152-148-153
Average 96 stars, based on 1 article reviews
cfx connect real time pcr machine biorad bio rad cfx96 real time pcr detection system - by Bioz Stars, 2026-11
96/100 stars
  Buy from Supplier
qrt pcr analysis  (Bio-Rad)
99
Bio-Rad qrt pcr analysis
FIGURE 1 YAP increases hTERT expression. A and B, MCF7 cells were transiently transfected with YAP-WT, YAP-5SA expression vectors or empty vector (Vec) as a control. Relative mRNA levels were examined by qPCR, and protein levels were detected by western blot. As a well-established YAP target gene, <t>CTGF</t> was used as a positive control. C and D, qPCR analysis of relative mRNA levels, and western blot analysis of protein levels in YAP-WT, YAP-5SA, or empty vector transfected HEK293A cells. E and F, WT and LATS1/2 KO HEK293A cells were transfected with control siRNA (siNC) or siRNAs against YAP (siYAP-1, siYAP-2) for 48 h. The mRNA levels were analyzed by qPCR, and protein levels were determined by western blot. Values represent mean ± SEM from three independent experiments. *P < .05, **P < .01, ***P < .001 by one-way ANOVA (A and C) or two-way ANOVA (E) with Fisher's LSD test. Relative hTERT protein levels were quantified with ImageJ software and normalized to β-actin, as indicated at the bottom of the blot
Qrt Pcr Analysis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/connect+qpcr+application+software/PrimePCR+Analysis+Software/10__3390_slash_agronomy11010018-82-8-14
Average 99 stars, based on 1 article reviews
qrt pcr analysis - by Bioz Stars, 2026-11
99/100 stars
  Buy from Supplier
gene exp ctgf mm01192932 g1  (Thermo Fisher)
97
Thermo Fisher gene exp ctgf mm01192932 g1
FIGURE 1 YAP increases hTERT expression. A and B, MCF7 cells were transiently transfected with YAP-WT, YAP-5SA expression vectors or empty vector (Vec) as a control. Relative mRNA levels were examined by qPCR, and protein levels were detected by western blot. As a well-established YAP target gene, <t>CTGF</t> was used as a positive control. C and D, qPCR analysis of relative mRNA levels, and western blot analysis of protein levels in YAP-WT, YAP-5SA, or empty vector transfected HEK293A cells. E and F, WT and LATS1/2 KO HEK293A cells were transfected with control siRNA (siNC) or siRNAs against YAP (siYAP-1, siYAP-2) for 48 h. The mRNA levels were analyzed by qPCR, and protein levels were determined by western blot. Values represent mean ± SEM from three independent experiments. *P < .05, **P < .01, ***P < .001 by one-way ANOVA (A and C) or two-way ANOVA (E) with Fisher's LSD test. Relative hTERT protein levels were quantified with ImageJ software and normalized to β-actin, as indicated at the bottom of the blot
Gene Exp Ctgf Mm01192932 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/connect+qpcr+application+software/Gene+Exp%2E+Ctgf%2C+Mm01192932_g1/10__1074_slash_jbc__m116__724138-229-93--1
Average 97 stars, based on 1 article reviews
gene exp ctgf mm01192932 g1 - by Bioz Stars, 2026-11
97/100 stars
  Buy from Supplier

Image Search Results


FIGURE 1 YAP increases hTERT expression. A and B, MCF7 cells were transiently transfected with YAP-WT, YAP-5SA expression vectors or empty vector (Vec) as a control. Relative mRNA levels were examined by qPCR, and protein levels were detected by western blot. As a well-established YAP target gene, CTGF was used as a positive control. C and D, qPCR analysis of relative mRNA levels, and western blot analysis of protein levels in YAP-WT, YAP-5SA, or empty vector transfected HEK293A cells. E and F, WT and LATS1/2 KO HEK293A cells were transfected with control siRNA (siNC) or siRNAs against YAP (siYAP-1, siYAP-2) for 48 h. The mRNA levels were analyzed by qPCR, and protein levels were determined by western blot. Values represent mean ± SEM from three independent experiments. *P < .05, **P < .01, ***P < .001 by one-way ANOVA (A and C) or two-way ANOVA (E) with Fisher's LSD test. Relative hTERT protein levels were quantified with ImageJ software and normalized to β-actin, as indicated at the bottom of the blot

Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Article Title: Human telomerase reverse transcriptase is a novel target of Hippo-YAP pathway.

doi: 10.1096/fj.201902147R

Figure Lengend Snippet: FIGURE 1 YAP increases hTERT expression. A and B, MCF7 cells were transiently transfected with YAP-WT, YAP-5SA expression vectors or empty vector (Vec) as a control. Relative mRNA levels were examined by qPCR, and protein levels were detected by western blot. As a well-established YAP target gene, CTGF was used as a positive control. C and D, qPCR analysis of relative mRNA levels, and western blot analysis of protein levels in YAP-WT, YAP-5SA, or empty vector transfected HEK293A cells. E and F, WT and LATS1/2 KO HEK293A cells were transfected with control siRNA (siNC) or siRNAs against YAP (siYAP-1, siYAP-2) for 48 h. The mRNA levels were analyzed by qPCR, and protein levels were determined by western blot. Values represent mean ± SEM from three independent experiments. *P < .05, **P < .01, ***P < .001 by one-way ANOVA (A and C) or two-way ANOVA (E) with Fisher's LSD test. Relative hTERT protein levels were quantified with ImageJ software and normalized to β-actin, as indicated at the bottom of the blot

Article Snippet: Primary antibodies against hTERT (ab32020; abcam), YAP (sc-101199; Santa Cruz Biotechnology), phospho-YAP (Ser127) (4911S; Cell Signaling Technology), LATS1 (3477T; Cell Signaling Technology), LATS2 (5888T; Cell Signaling Technology), TEA domain family transcription factor 4 (TEAD4) (ab58310; abcam), connective tissue growth factor (CTGF) (sc-365970; Santa Cruz Biotechnology), and β-Actin (M1210-2; HuaAn Biotechnology) were used in this study.

Techniques: Expressing, Transfection, Plasmid Preparation, Control, Western Blot, Positive Control, Software

FIGURE 3 YAP transcriptionally activates the hTERT promoter by interacting with TEAD. A and B, YAP induces hTERT expression in a TEAD-dependent manner. hTERT mRNA and protein levels were determined in HEK293A cells transfected with empty vector or YAP expressing vectors as indicated. Relative hTERT protein levels were quantified with ImageJ software and normalized to β-actin, as indicated at the bottom of the blot. C, HEK293A cells were transiently transfected with YAP-WT, 5SA, S94A expression vectors or empty vector, along with luciferase reporter vector containing a region of hTERT promoter (−951 to +58 bp). Luciferase activity was detected at 48 h after transfection. D, Luciferase reporter assay in HEK293A cells transfected with control (pGL2-Basic), hTERT promoter fragment (phTERT wt), or hTERT promoter fragment carrying mutation in potential TEAD binding motif (phTERT-TEAD mut), along with YAP-5SA expression vector. E, ChIP-qPCR analysis of YAP and TEAD4 enrichment at different regions of hTERT promoter in LATS1/2 KO HEK293A cells. GAPDH promoter was used as a negative control, and promoter of YAP target gene CTGF was used as a positive control. Values represent mean ± SEM from at least three independent experiments. *P < .05, **P < .01, ***P < .001 by one-way ANOVA (A, C and D) or two-way ANOVA (E) with Fisher's LSD test

Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Article Title: Human telomerase reverse transcriptase is a novel target of Hippo-YAP pathway.

doi: 10.1096/fj.201902147R

Figure Lengend Snippet: FIGURE 3 YAP transcriptionally activates the hTERT promoter by interacting with TEAD. A and B, YAP induces hTERT expression in a TEAD-dependent manner. hTERT mRNA and protein levels were determined in HEK293A cells transfected with empty vector or YAP expressing vectors as indicated. Relative hTERT protein levels were quantified with ImageJ software and normalized to β-actin, as indicated at the bottom of the blot. C, HEK293A cells were transiently transfected with YAP-WT, 5SA, S94A expression vectors or empty vector, along with luciferase reporter vector containing a region of hTERT promoter (−951 to +58 bp). Luciferase activity was detected at 48 h after transfection. D, Luciferase reporter assay in HEK293A cells transfected with control (pGL2-Basic), hTERT promoter fragment (phTERT wt), or hTERT promoter fragment carrying mutation in potential TEAD binding motif (phTERT-TEAD mut), along with YAP-5SA expression vector. E, ChIP-qPCR analysis of YAP and TEAD4 enrichment at different regions of hTERT promoter in LATS1/2 KO HEK293A cells. GAPDH promoter was used as a negative control, and promoter of YAP target gene CTGF was used as a positive control. Values represent mean ± SEM from at least three independent experiments. *P < .05, **P < .01, ***P < .001 by one-way ANOVA (A, C and D) or two-way ANOVA (E) with Fisher's LSD test

Article Snippet: Primary antibodies against hTERT (ab32020; abcam), YAP (sc-101199; Santa Cruz Biotechnology), phospho-YAP (Ser127) (4911S; Cell Signaling Technology), LATS1 (3477T; Cell Signaling Technology), LATS2 (5888T; Cell Signaling Technology), TEA domain family transcription factor 4 (TEAD4) (ab58310; abcam), connective tissue growth factor (CTGF) (sc-365970; Santa Cruz Biotechnology), and β-Actin (M1210-2; HuaAn Biotechnology) were used in this study.

Techniques: Expressing, Transfection, Plasmid Preparation, Software, Luciferase, Activity Assay, Reporter Assay, Control, Mutagenesis, Binding Assay, ChIP-qPCR, Negative Control, Positive Control